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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer

Fig. 2

E-cadherin high DU145 sub-line presents higher levels of CXCR3 and CXCR3-B. In (a-g), DU145 sub-lines with low E-cadherin (DU-L) or high E-cadherin (DU-H). a Immunofluorescence staining of E-cadherin (green) and DAPI (blue). Bar = 50μm. b Immunoblot of E-cadherin expression, GAPDH as loading control. c Quantitative real-time PCR analysis of mRNA levels of CXCR3-A, CXCR3-B and E-cadherin; normalized to GAPDH. In (d-g), flow cytometry assay of whole cell level of CXCR3-B (d), whole cell level of total-CXCR3 (e), externally-accessible CXCR3-B (f), externally-accessible total-CXCR3 (g). The Geometric Mean Fluorescence Intensity (MFI) is on the right panel. Student t-test, *, p < 0.05; ****, p < 0.0001, N.S., non-specific. One representative experiment, of at least 3 independent repeats, is presented in all panels

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