Fig. 1
From: Expression of E-cadherin and specific CXCR3 isoforms impact each other in prostate cancer
Membrane-presented CXCR3-B is increased in epithelial PCa cells. In (a-g), DU145 cells treated with 500 nM PD153035 for 48 h to induce epithelial conversion (PD(MErT)), DMSO was added as control. a Immunofluorescence staining of E-cadherin (green) and DAPI (blue). Bar = 25μm. b Immunoblot of E-cadherin expression, GAPDH as loading control. c) Quantitative real-time PCR analysis. Relative mRNA levels of CXCR3-A, CXCR3-B in DU145 cells (left panel); and CXCR3-A, CXCR3-B and E-cadherin in epithelial converted cells (fight panel); normalized to GAPDH. In (d-g), flow cytometry assessments of whole cell level of CXCR3-B (d), whole cell level of total-CXCR3 (E), externally-accessible CXCR3-B (F), externally-accessible total-CXCR3 (g). The Geometric Mean Fluorescence Intensity(MFI) is on the right panel. Student t-test, **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. One representative experiment of at least 3 independent repeats is presented in all panels