Table 1 Specifications of primary and secondary antibodies employed for western blotting
From: N-methyl-D-aspartate (NMDA) receptor expression and function is required for early chondrogenesis
Primary antibody | Supplier | Catalogue number | Dilution | Secondary antibody | Supplier | Catalogue number | Dilution |
|---|---|---|---|---|---|---|---|
Sox9 | Abcam | ab26414 (polyclonal) | 1:600 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
P-Sox9 | Merck | HPA001758 (polyclonal) | 1:600 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
GluN1 | Cell Signaling | 4204 | 1:600 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
GluN2A | Abcam | ab14596 | 1:600 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
GluN2B | Cell Signaling | 4207 | 1:600 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
GluN3A | Merck/Millipore | 07–356 | 1:200 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
GluN3B | Abcam | ab35677 | 1:200 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |
GAPDH | Merck/Millipore | AB2302 | 1:1500 | Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | Bio-Rad | 1,706,515 | 1:1000 |