Fig. 3
From: N-methyl-D-aspartate (NMDA) receptor expression and function is required for early chondrogenesis
Effect of locally administered 20 μM NMDA on the cytosolic Ca2+ levels in Fura-2-loaded cells in micromass cultures at room temperature. a. Ca2+ transients evoked by administration of NMDA in cells on different days of culturing. When not indicated, Ca2+ transients were measured in the presence of 1.8 mM [Ca2+]e. Representative records of 3 independent experiments. Lines under graphs indicate the application of NMDA, KCl (120 mM) or Ca2+-free Tyrode’s. b. Changes in the peak amplitude of NMDA-evoked Ca2+ transients (average ± SEM) detected on different days of culturing in Fura-2-loaded cells. Numbers above bars represent the ratio of cells responding to the NMDA challenge. Representative data of 3 independent experiments, each showing similar trends of changes. c. Record showing the lack of Ca2+ transients evoked by administration of 10 μM glycine measured in the presence of 1.8 mM [Ca2+]e. Representative record of 3 independent experiments