Fig. 9

Expression and function of FGFR3 in TNBC cell lines exhibiting low-moderate FGFR3 phosphorylation. a, FGFR3 expression analysed by immunoprecipitation and Western blot. Lysates from CAL51, MFM223 and MDA-MB-231 cells were subjected to immunoprecipitation of wildtype FGFR3, which was then detected by Western blotting using the FW FGFR3 antibody. IgG was used as a negative control for immunoprecipitation. TCL = total cell lysate. UF = unbound fraction. b, Confirmation of FGFR3 expression by knockdown. Cell lines from (A) were subjected to FGFR3 knockdown prior to immunoprecipitation and Western blot analysis. KD = knockdown. c, Effect of FGFR3 knockdown on cell proliferation. Cells were transfected with 20 nM of individual siRNAs targeting wildtype FGFR3 (W1–2) and cell proliferation indirectly assayed via a MTS absorbance assay. Error bars: mean ± standard error, of three biological replicates