Fig. 7

Dose dependent effect of the FGFR inhibitor PD173074 on FGFR3 downstream signaling pathways in the SUM185PE cell line. a, Expression/activation of downstream signaling proteins 1 h post-treatment with the indicated doses of PD173074. Arrow indicates FGFR3-TACC3 fusion protein, bracket indicates wildtype FGFR3. b, Quantification by densitometry of (A). Data were first normalized relative to the tubulin control, then phosphorylated proteins were normalized to total protein, finally data were expressed relative to the DMSO control which was arbitrarily set at 1.0. Error bars: mean ± standard error, of three biological replicates. ** indicates p-value of < 0.01,*** < 0.001