Fig. 3
From: FGFR3 signaling and function in triple negative breast cancer
Localization of FGFR3-TACC3 fusion and wildtype FGFR3 by immunofluorescent staining in SUM185PE cells. SUM185PE cells were fixed and permeabilised then immunostained with a, wildtype FGFR3 antibody or b, FW FGFR3 antibody detecting both FGFR3-TACC3 fusion and wildtype FGFR3. Dapi was used to stain DNA of the cells. Images were obtained by confocal microscopy and are representative of 3 biological replicates, each involving analysis of at least 10 cells. c and d, representative images for immunostaining with the wildtype FGFR3 antibody in mitotic SUM185PE cells. For spindle visualisation, SUM185PE cells were treated with 3 mM of thymidine to halt cell cycle progression at the G1/S phase, and then released into complete media to allow cells to undergo mitosis. Tubulin immunostaining was used to visualize the mitotic spindle