Fig. 5

DCX Nuclear Import contributes to GBM development. a Wound healing migration assay comparing DCX⁺ U251MG cells with DCX NLS2-mutant cells. t1=3.118, df1=4, t2=8.655, and df2=4. b Boyden’s transwell invasion assay involving DCX⁺ vector cells and DCX NLS2-mut cells, quantitated by Image-Pro-Plus. t1=4.680, df1=4, t2=8.868 df2=4. c Similar experiments were performed with GDCX-C6 cells treated with GTP analogs. F1=13.508, df1=2, F2=50.392, df2=2. d Hematoxylin-Eosin staining of enlarged mice brain section showing infiltrating tumors at the invasive frontiers. The dashed boundary depicts the distance covered by invasive cells. Scale bars, 50μm. e Representative immunohistochemical staining to compare GFAP (df=4, t=5.099), or vimentin (df=4, t=6.622) immunoreactivity in vector and DCX NLS2-mut tumors. Scale bars, 25μm. f Immunostaining of brain sections showing DCX nuclear accumulation intensity. Scale bars=25μm. g Immunofluorescence image of invasive glioma markers (CD31 and EGFR) in DCX-vector and DCX NLS2-mut tumors. Scale bar, 25μm