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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression

Fig. 3

DCX nuclear import requires a classical nuclear localization signal. a Schematics of putative DCX NLS position and sequence as well as their structural position in the cartoon representation of DCX N-terminal region (suspension point indicates the extended part of DCX NLS2). b Illustration of DCX truncates containing putative DCX NLS mutants fused with GFP. Black bars indicate the location of NLS; blank spaces indicate deleted corresponding putative NLSs. c Subcellular localization of DCX in stable U251MG cells expressing DCX NLS mutant compared with DCX+ vector as detected by immunofluorescence. Scale bar 20 μm. d DCX subcellular localization levels in mutant cells as detected by western blotting. F = 67.156 and df = 5. e Co-immunoprecipitation of DCX with importin-α and importin-β in DCX mutants and vector control cells. f Pulse shape analysis of U251MG cells expressing DCX mutants compared with the vector showing lower pulse height in DCXNLS2 mutant cells

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Cell Communication and Signaling

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