Fig. 2

DCX nuclear import occurs via the RanGTPase dependent pathway. a Formation of protein-protein interaction of DCX with endogenous importin-α and importin-β by co-immunoprecipitation, Rabbit IgG was loaded in the second lane as the control immunoprecipitation. b Subcellular localization of DCX from GDCX-C6 cells treated with GTP analogs. F = 60.328 and df = 2. c Immunostaining of GDCX-C6 cells treated with GTPγS and GDPβS. Scale bar, 20 μM. d Schematics showing the principles of pulse shape analysis (PulSA), non-aggregated fluorescent protein generates a higher pulse height than inclusion body. e Cytograms showing pulse height of GTPγS-treated and untreated GDCX cells, upper panels. Histographs of analyzed data generated from flow cytometry experiments (in arbitrary units, a.u.), lower panels. Data are presented as Mean ± SEM. GDCX 0 min vs. GDPβS 30 min treatment (P = 0.0032, t = 2.964 df = 465.8), GDCX 0 min vs GDPβS 1 h treatment (P = 0.0031, t = 2.97 df = 541), GDCX 0 min vs GTPγS 30 mins treatment (P = 0.0001, t = 4.695 df = 882.6), GDCX 0 min vs GTPγS 1 h treatment (P = 0.0001, t = 4.595 df = 897). f Co-immunoprecipitation of DCX with importin-α and importin-β in whole-cell lysates of GTPγS, GDPβS, and untreated GDCX-C6 cells