Fig. 6

TRIM30 and miR-595 regulate Sox17 in a mutually independent manner. (a) TPC-1 cells were transfected with plasmids and miRNAs as indicated for 48 h prior to luciferase activity analyses. (b) Experiments were performed as in (a), except the shRNA-TRIM30 (50 pmol) were used. (c and d) Experiments were performed as in (a and b), except the indicated anti-miR-595 or anti-miR-control were used. (E) TPC-1 cells were transfected with the plasmids and miRNAs as indicated for 32 h. Co-IP and immunoblot analyses were performed with the indicated antibodies (left panel) and qRT-PCR was performed with the indicated primers. (f) Experiments were performed as in (e), except the indicated anti-miR-595 or anti-miR-control were used. All experiments were repeated at least three times. Bar graphs present means ± SD, n = 3 (**P < 0.01; *P < 0.05)