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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: TRIM30 modulates Interleukin-22-regulated papillary thyroid Cancer cell migration and invasion by targeting Sox17 for K48-linked Polyubiquitination

Fig. 4

IL-22 regulate β-catenin signaling via TRIM30/Sox17 axis. (a) TPC-1 cells were treated with a concentration of 50 ng/ml rhIL-22 for the indicated time points. β-catenin levels were quantified by qRT-PCR (upper panel) and western blot (lower panel). (b) TPC-1 cells were treated with rhIL-22 for 24 h at indicated concentrations. β-catenin levels were quantified by qRT-PCR (upper panel) and western blot (lower panel). (c) TPC-1 cells transfected with the indicated plasmid for 24 h and treated with or without 50 ng/ml rhIL- 22 for 24 h. Co-IP and immunoblot analyses were performed with the indicated antibodies. (d) TPC-1 cells transfected with the indicated plasmid for 24 h and treated with or without 50 ng/ml rhIL- 22 for 24 h prior to luciferase assays. (e) Experiments were performed as in (d), except cells were transfected with shRNA-Sox17. (f) TPC-1 cells transfected with the indicated plasmid for 24 h and treated with or without 50 ng/ml rhIL- 22 for 24 h. Cytosolic and nuclear extracts were prepared and subjected to western blot analyses. Lamin A and β-actin were used as markers for nuclear and cytosolic fractions, respectively. (g) Experiments were performed as in (f), except cells were transfected with shRNA-Sox17. (h) Experiments were performed as in (c), except TRIM−/− cells were used. (i and j) Experiments were performed as in (d and e), except pCMV-TRIM30 (i) or shRNA-TRIM30 (j) were used. (k and l) Experiments were performed as in (f and g), except pCMV-TRIM30 (k) or shRNA-TRIM30 (l) were used. All experiments were repeated at least three times. Bar graphs present means ± SD, n = 3 (**P < 0.01; *P < 0.05)

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