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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: TRIM30 modulates Interleukin-22-regulated papillary thyroid Cancer cell migration and invasion by targeting Sox17 for K48-linked Polyubiquitination

Fig. 3

IL-22 promotes cell growth and motility via TRIM30/Sox17 axis. (A) TPC-1 cells transfected with the pCMV-TRIM30 or control vector for 24 h and treated with or without 50 ng/ml rhIL- 22 for 24 h prior to cell proliferation assay. (B) Experiments were performed as in (a), except cells were transfected with shRNA- TRIM30. (C and D) TPC-1 cells were transfected with the indicated plasmid, then treated with rhIL-22 (50 ng/ml) for 48 h prior to Transwell migration (c) or invasion (d) assays. (e and f) Experiments were performed as in (c and d), except cells were transfected with shRNA-TRIM30. (G) TPC-1 cells were transfected with the plasmids as indicated for 48 h prior to cell proliferation assay. (i) Experiments were performed as in (g), except cells were transfected with shRNA-Sox17. (I and J) TPC-1 cells were transfected with the plasmids as indicated for 48 h prior to Transwell migration (i) or invasion (j) assays. (k and l) Experiments were performed as in (I and J), except cells were transfected with shRNA-Sox17. (M-P) TPC-1 cells were stably expressing TRIM30, SOX17, or TRIM30 and SOX17 were subcutaneous injected into nude mice. Shown are stripped tumors (m), the growth curve (n), and the average weight (o). The indicated tumor tissue from a single mouse in each group was cut into several pieces and then used for immunoblot (p). Tumors were from the fourth column in each group. Bar graphs present means ± SD, n = 3 (**P < 0.01; *P < 0.05)

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