Fig. 1

TRIM30 associates with Sox17. (a) TPC-1 cells were transfected with vector control or Myc-Sox17 for 48 h. Cells were lysed and immunopurified with anti-Flag affinity columns and eluted with Flag peptide. The eluates were resolved using SDS-PAGE and were silver-stained. The various protein bands were retrieved and analyzed using mass spectrometry. (b) 293 T cells were transfected with Flag-tagged TRIM30 (Flag-TRIM30) and Myc-tagged Sox17 (Myc- Sox17). Forty-eight hours post-transfection, Co-IP and immunoblot analysis were performed with the indicated antibodies. (c) Experiments were performed in similar fashion to those in (b), except DNAase was used. (d) Experiments were performed in similar fashion to those in (b), except Flag-TRIM8 was used. (e) Schematic diagram of the full-length and truncated constructs of Sox17 (upper panel). 293 T cells were co-transfected with Flag-TRIM30 and the indicated truncated Sox17 constructs for 48 h. Co-IP and immunoblot analyses were performed with the indicated antibodies (lower panel). (f) Experiments were performed in similar fashion to those in (e), except indicated truncated constructs of TRIM30 were used. (g) TPC-1 cells were treated with rhIL-22 at 50 ng/ml for indicated times. Immunoprecipitation and immunoblot analysis were performed with the indicated antibodies. All experiments were repeated at least three times