Fig. 6
Mutation of HtrAHp has no effect on the secretion of proteins. Proteolytic activity of the HtrAHp protein is important in the process of secretion of this protein outside the bacterial cell. Total proteins were labelled as a pellet, bacteria-free secreted samples was labelled as a supernatant. a Western blotting analysis of H. pylori lysates. The CagA protein was used as a control (as a non-secreted protein). b The band intensities of secreted proteins were quantified densitometrically using 1Dscan Ex program and the relative amount of secreted protein is given. Significant differences were analyzed using Bonferroni test (***p < 0.001). The standard error of mean (SEM) was calculated using at least three repetitions. Immunodetection using Lon antibodies was performed in duplicate