Fig. 6

USP22 knockout sensitizes NSCLC cells to irradiation. Representative flow cytometry profile of apoptotic cells in a. The parent (upper panel) and USP22−/− A549 (lower panel) cancer cells; and b. The parent (upper panel) and USP22−/− H1299 cancer cells (lower panel). Cells were first subjected to 5 or 10 Gy irradiation; apoptosis was measured at 48 h post-irradiation. Quantitative analysis of the experiments shows that apoptotic cells were significantly increased in both USP22−/− A549 (a, right panel) and USP22−/− H1299 cancer cells (b, right panel) compared with their parent cells (USP22+/+). The experiment was repeated three times and data represent the average of the early apoptotic and late apoptotic cells (** P < 0.01). The dynamics of γ-H2AX, H2Bub1, and apoptotic markers PARP cleaved product (c-PARP for cleaved protein) in c. the parent and USP22−/− A549 cancer cells, and d. the parent and USP22−/− H1299 cancer cells at 2 h, 48 h post-irradiation that analyzed by Western blot