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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Ubiquitin-specific protease 22 is critical to in vivo angiogenesis, growth and metastasis of non-small cell lung cancer

Fig. 4

Effects of USP22 knockout on metastasis and survival of metastatic cancer-bearing mice. a. NSG mice were injected with 2 × 106 of the parent (USP22+/+) or USP22−/− A549 cancer cells (n = 10) through tail vein. Four weeks after injection, mice were killed, lungs (left panel) were excised and weighted (right panel), ** P < 0.01 compared with A549 cells. b. E-cadherin IHC and H&E stains of lung metastasis formed by A549 (USP22+/+) (left panel) or USP22−/− cancer cells. H&E shows almost 100% area of lung was occupied by A549 metastasis, while less than 5% (blue arrow area) was occupied by USP22−/− A549 cancer cells (magnification, × 200). c. Kaplan-Meier survival curves of mice bearing the parent and USP22−/− cancer cells (P < 0.0001). d. NSG mice were injected with 2 × 106 of the parent (USP22+/+) or USP22−/− H1299 cancer cells (n = 10) through tail vein. Four weeks after injection, mice were killed, livers (left panel) were excised and weighted (right panel), ** P < 0.01 compared with the parent H1299 cancer cells. e. E-cadherin IHC and H&E stains of lung metastasis formed by the parent (left panel) or USP22−/− H1299 cancer cells (right panel) (magnification, × 200). f. Kaplan-Meier survival curves of mice bearing the parent and USP22−/− H1299 cancer cells, ** P < 0.01 compared with the parent H1299 cancer cells

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