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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Ubiquitin-specific protease 22 is critical to in vivo angiogenesis, growth and metastasis of non-small cell lung cancer

Fig. 2

Alternated gene expression and signaling pathways in A549 and H1299 cells upon USP22 knockout. a. qRT–PCR analysis of selected differentially expressed genes in USP22−/− A549 and USP22−/− H1299 cancer cells compared to the parent cells. The level of each gene in USP22−/− cells is the average ratio of triplicate samples, and is presented as the ratio to the parent cancer cells (USP22+/+); P < 0.05, compared with the parent cells. b. Selected signaling pathways that were enriched in USP22−/− A549 and USP22−/− H1299 cancer cells (P < 0.05, FDR < 5%). c. Western blot analysis of differentially expressed ALDH1A3, Cyclin D1, Cyclin D2, Cyclin E2, c-Myc, and SETD7 in USP22−/− cancer cells. c. Western blot analysis of AKT, ERK, E-Cadherin, Vimentin, p53, p16, Sirt1 in the USP22−/− and the parent cancer cells

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