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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Carbonic anhydrase 2 (CAII) supports tumor blood endothelial cell survival under lactic acidosis in the tumor microenvironment

Fig. 5

Tumor-derived factors induce CAII upregulation in endothelial cells. a Human NECs (hNECs) were treated with tumor or control-conditioned medium for 24 h. CAII mRNA expression was determined by RT-qPCR. b hNECs were stimulated with 20 ng/mL VEGF for 24 h, and RNA was isolated. CAII expression was determined by RT-qPCR. (C) hNECs were exposed to tumor-CM or control CM containing 10 μM Ki8751 (VEGFR2 inhibitor) for 24 h. CAII expression was determined by RT-qPCR. d hNECs were stimulated with 20 ng/mL VEGF alone or in combination with 10 μM Ki8751 (VEGFR2 inhibitor) or 2 mg/mL bevacizumab (VEGF neutralizing antibody). The cells were cultured for 36 h, and the protein was collected for analysis by western blotting. All data presented as mean ± SD, *P < 0.01, **P < 0.001, by two-tailed unpaired Student’s t-test, n = 3

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