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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Carbonic anhydrase 2 (CAII) supports tumor blood endothelial cell survival under lactic acidosis in the tumor microenvironment

Fig. 4

TECs are more sensitive to carbonic anhydrase II inhibition. a, b MCT1 and CAII inhibition by siRNA knockdown in TECs was confirmed by RT-qPCR. c The proliferation of MCT1 and CAII knockdown TECs in complete medium measured by the MTS assay. The values refer to fold change relative to control siRNA-transfected cells. d, e MCT1 and CAII knockdown TECs were exposed to lactosis (20 mM lactate, pH 7.3) and lactic acidosis (20 mM lactate, pH 6.9), 24 h after knockdown. Cell proliferation was determined by the MTS assay. The values refer to the fold change relative to control siRNA-transfected cells. Results presented as mean ± SD, *P < 0.01, **P < 0.001; N.S., not significant, n = 3

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Cell Communication and Signaling

ISSN: 1478-811X