Fig. 2

MORC2 is required for altered nucleosome stability after DNA damage. a HeLa and MCF-7 cells were treated with or without 8 μM CPT for the indicated times and analyzed by immunoblotting with the indicated antibodies. b HeLa and MCF-7 cells were treated with or without 8 μM CPT for 1 h. Nuclei were isolated and subjected to salt solubilization assay as described in Fig. 1b and c. NPM and hnRNPM are shown as loading controls. c WT and MORC2 KO HeLa or MCF-7 cells were treated with 8 μM CPT for 1 h. The isolated nuclei were subjected to salt solubilization assay as described above. NPM and hnRNPM are shown as loading controls