Fig. 5

Phosphorylation map of DVL3. All identified phosphorylation sites obtained from the pipeline #2 are visualized as a heatmap. Color intensities reflect relative change in the site phosphorylation (red – decrease, green – increase). Following additional information is also provided: a) Corresponding sites in human DVL1 and DVL2. Positions conserved in DVL1 and/or DVL2 either as Ser or Thr are highlighted in yellow. Position of the structured domains (DIX, PDZ and DEP) is indicated. b) The sequence of the phosphorylated epitope. Five amino acids before and after the identified phosphorylated site are shown. c) Mean absolute intensities of the phosphorylation sites in the control (DVL3 without exogenous kinase; N = 6) are expressed in the shades of blue. Numbers indicate decadic logarithm of the mean. ND indicated in white corresponds means “not detected”. All signals lower than 1 × 10⁶, corresponding to log value 6.0 were considered as not detected. d) Nine columns represent heat map of relative change of phosphorylated peptide intensities (in log10 scale) obtained for individual kinases (relative to control). Numbers in the heatmap fields (0, 1, 2, 3) indicate the number of experimental replicates with the positive identification of the given phosphorylated site. e) Grey boxes indicate the position of clusters of sites analyzed also according to the pipeline #3 in the Additional file 2: Figure S2