Fig. 2
From: Comparative phosphorylation map of Dishevelled 3 links phospho-signatures to biological outputs
Validation of the panel of DVL3 kinases. a: Visualization of the kinases used in this study in the phylogenetic kinome tree (http://www.kinhub.org/kinmap/). The individual kinases are representatives of distant kinase groups except for CK1ε and TTBK2 that are members of CK1 superfamily. b. HEK293 cells were transfected by plasmids encoding FLAG-DVL3 and the indicated kinase. Ability of individual kinases to promote DVL3 phosphorylation detected as the electrophoretic mobility shift on WB was assayed. Alpha-tubulin was used as a loading control