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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: TPP-related mitochondrial targeting copper (II) complex induces p53-dependent apoptosis in hepatoma cells through ROS-mediated activation of Drp1

Fig. 6

CTB promotes activation of Drp1 and mitochondrial aggregation by promoting ROS production. Cells were treated with CTB at the indicated concentrations (0, 1, 2, 4 μΜ) for 24 h. a Micrographs of mitochondrial morphology visualized by Mito-Tracker Green probe. Scale bar: 10 μm. b Western blot analysis of p-Drp1 (Ser616)/Drp1 expression. c Western blot analysis of Drp1 expression in cytoplasm or mitochondria respectively. d The molecular simulation used human Drp1 crystal (3W6P) as the acceptor, and the docking of CTB and 3W6P was detected by Gold docking software. The cyan small molecule represents the original ligand and the research molecule (CTB); the green dotted line indicated the intermolecular van der Waals force and the magenta dotted line indicated the positive Ion-Pi interaction. e Western blot analysis of p-Drp1 (Ser616)/Drp1 expression in SMMC-7721 cells treated with CTB (2 μM) and/or NAC (10 mM) for 24 h. f Western blot analysis of Drp1 expression in cytoplasm or mitochondria respectively. Data are represented as mean ± SD. Significance: *P < 0.05, **P < 0.01 and ***P < 0.001 vs Control; #P < 0.05, ##P < 0.01 and ###P < 0.01 vs CTB (2 μΜ) treatment

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