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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: TPP-related mitochondrial targeting copper (II) complex induces p53-dependent apoptosis in hepatoma cells through ROS-mediated activation of Drp1

Fig. 2

CTB destroyed mitochondrial structure and function of hepatoma cells. SMMC-7721 cells were treated with different CTB concentrations (0, 1, 2, 4 μM) for 24 h. a Janus green staining for detecting mitochondrial activity. Representative photographs were shown. Scale bar: 50 μm b Observation of mitochondrial morphology by Transmission Electron Microscope (TEM). Scale bar: 0.2 μm. c Effect of CTB on MMP (ΔΨm) was determined in SMMC-7721 cells by JC-1 staining. Scale bar: 50 μm. d The opening of mPTP was detected by Rhodamine123 staining. Scale bar: 50 μm. e ATP generation was detected by ATP Detection Kit. Scale bar: 50 μm. Scale bar: 50 μm. Data are represented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 vs Control

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Cell Communication and Signaling

ISSN: 1478-811X