Fig. 3
From: Loss-of-function phenotype of a PKCθT219A knockin mouse strain
TCR-dependent activation signals lead to a strong defect in IL-2 production both in the peripheral CD4+ and CD8+ T cell subsets. a and d, proliferative responses of peripheral MACS-sorted CD4+ and CD8+ T cells after TCR stimulation revealed a partial defect in the knockin animals similar to responses in PKCθ-deficient mice. T cells isolated from heterozygous animals show normal proliferation comparable to the wild-type controls. b and e, PKCθT219A CD4+ and CD8+ T cells show a robust and highly reproducible defect in IL-2 secretion response upon stimulation with CD3/CD28 antibodies, which is reminiscent of the PKCθ knockout T cells. C and F IFN-γ levels were reduced both in knockin and knockout T cells whereas the heterozygous genotype showed a mostly unaffected IFN-γ secretion, as revealed by Bioplex measurements. Shown are the mean values of at least three independent experiments ± SEM (a-f). Unpaired Students t-test was used for statistics