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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Loss-of-function phenotype of a PKCθT219A knockin mouse strain

Fig. 2

a and b a detailed flow cytometric analysis of cellularity of thymus and spleen from wild-type, knockin and knockout mice revealed a normal tissue distribution of T and B cells in the T219A knockin mice, demonstrating intact thymocyte development and tissue distribution in the periphery. Total thymocyte and spleen counts were determined ex vivo using a Luna cell counter. Graphs summarizing three experiments are shown (the gating strategy and representative FACS dot blots are shown in Additional file 1: Figure S1). Data are shown as percent positive cells of total lymphocytes. c, the surface expression of CD25, CD44, and CD69 on overnight CD3/CD28 activated CD4+ T cell populations did not reveal any gross differences between the knockin mice and the wild-type controls. Data are shown as mean fluorescence intensities ± SEM (n = 3). Statistical analyses were performed using students t-test

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