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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Platelets promote breast cancer cell MCF-7 metastasis by direct interaction: surface integrin α2β1-contacting-mediated activation of Wnt-β-catenin pathway

Fig. 5

Both Wnt-β-catenin and TGF-β1/pSmad3 pathways promote MCF-7 cell EMT. a ChIP assay was performed to determine the combination between β-catenin/pSmad3 and the promoter of Snail and Slug. b The IP assay was performed to confirm the binding between β-catenin and pSmad3. c The promoter activity of Snail and Slug before and after the co-incubation with or without adding XAV, an inhibitor for β-catenin or SB, an inhibitor for pSmad3 pathway. d The mRNA expression of EMT markers was assessed in MCF-7 cells after the direct contacting and the transwell assay. *p < 0.05, **p < 0.01, ##p < 0.01

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