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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Platelets promote breast cancer cell MCF-7 metastasis by direct interaction: surface integrin α2β1-contacting-mediated activation of Wnt-β-catenin pathway

Fig. 2

Surface integrin α2β1 mediated the contacting between platelet and MCF-7 cells. MCF-7 cells contacted with fluorescein-labeled platelets or pellets by the co-incubation for 30 min. a The percentage of fluorescein-positive MCF-7 cells was analyzed by the flow cytometry. Fluorescein-labeled pellets were co-incubated with MCF-7 cells after their surface proteins being inhibited by anti-P-selectin, anti-AK7, and anti-CRC64, the antibodies against P-selectin, integrin α2β1 and integrin αIIbβ3, respectively. b The relative mRNA levels of EMT markers in MCF-7 cells were determined by qRT-PCR and c the percentage of fluorescein-positive MCF-7 cells was analyzed by the flow cytometry. d The percentage of surface integrins on the MCF-7 cells. MCF-7 cells were co-incubated with fluorescein-labeled pellets after their surface proteins being inhibited by anti-AK7 and anti-CRC64. e The relative mRNA levels of EMT markers in MCF-7 cells were determined by qRT-PCR, and f the percentage of fluorescein-positive MCF-7 was analyzed by the flow cytometry. *p < 0.05, **p < 0.01, ##p < 0.01

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Cell Communication and Signaling

ISSN: 1478-811X