Fig. 5

Lack of PIM1 target sites reduces the ability of NFATC1 to promote migration of prostate cancer cells. Wild-type (WT), double mutant (DM) or multi mutant (MM) NFATC1 were transiently expressed in PC-3 cells (a) or DU-145 cells (b). For wound healing assays, cell layers were scratched 24 h after transfection with a 10 μl pipette tip and the wounded areas were allowed to recover for another 24 h in the presence of either DMSO or 10 μM DHPCC-9. Shown are representative pictures taken at 0 h and 24 h time-points, and average wound healing percentages