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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Phosphorylation of NFATC1 at PIM1 target sites is essential for its ability to promote prostate cancer cell migration and invasion

Fig. 4

Effects of PIM-dependent phosphorylation on NFAT activity. a The expression levels of PIM1 and NFATC1 mRNAs were determined from our previously published RNA-sequencing dataset from PC-3, DU-145 and LNCaP cell lines [36]. b The impact of PIM-dependent phosphorylation on NFAT activity was analysed by luciferase assays in PC-3 and DU-145 cells that transiently expressed wild-type (WT) or multi mutant (MM) NFATC1. Cells were treated with either DMSO (−) or 10 μM DHPCC-9 (+). In addition, DU-145 cells had been pre-treated with TPA and IM. Shown are means of relative luciferase activities from two independent experiments with four parallel samples, the results of which had been normalized against the mock-transfected control samples. c Similar luciferase assays were performed also with cells treated with either EtOH (−) or 1 μM CsA (+)

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Cell Communication and Signaling

ISSN: 1478-811X