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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: ΗΙF1α, EGR1 and SP1 co-regulate the erythropoietin receptor expression under hypoxia: an essential role in the growth of non-small cell lung cancer cells

Fig. 5

Immunoprecipitation analysis of the protein-protein and protein-DNA interactions around proximal EPO-R promoter under hypoxia. a Nuclear co-immunoprecipitation of the transcription factors HIF1α, EGR1, and SP1. Nuclear lysates were pulled down by immunoprecipitation (IP), followed by western blotting (IB) detection with TFIID used an input control. The experiments were repeated at least twice. b Chromatin immunoprecipitation (ChIP) analysis of promoter DNA binding activity under hypoxia. The chromatins pull-downed by antibody against SP1 or EGR1, or by normal IgG were analyzed by standard PCR using primers for proximal EPO-R promoter (pEPO-R). βactin promoter primers (pActin) were used as an internal control. The experiments were repeated at least twice. c A diagram depicts how HIF1α, EGR1, and SP1 cooperatively and sequentially regulates EPO-R expression under hypoxia condition in cultured NSCLC cells

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Cell Communication and Signaling

ISSN: 1478-811X