Fig. 8
From: SUMO3 modification by PIAS1 modulates androgen receptor cellular distribution and stability
MDM2 is not required for SUMO3 modified PIAS1 induced AR nuclear export. DU145 cells were transiently co-transfected with flag-AR, SUMO3, myc-PIAS1 and control shRNA or MDM2 shRNA for 48 h. Cells were then fixed and stained with anti-MDM2 (green) and anti-flag (red) or anti-myc mouse monoclonal antibody (red). Nuclei were visualized by DAPI staining. Representative images of transfected cells were shown