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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: SUMO3 modification by PIAS1 modulates androgen receptor cellular distribution and stability

Fig. 3

Lysines 386 and 845 of AR are critical for PIAS1/SUMO3 overexpression induced nuclear export and degradation of AR. (a) DU145 cells cotransfected with myc-PIAS1, GFP-SUMO3 and wildtype AR or various point mutants of AR expression constructs as indicated for 48 h were subjected to immunoprecipitation with anti-AR antibody (IP), and this was followed by immunoblotting with anti-AR (IP) or anti-SUMO3 (IB) antibodies. (b) DU145 cells cotransfected with plasmids as described in A were fixed and stained with anti-flag mouse monoclonal antibody, then PI-conjugated anti-mouse IgG antibody (red). Nuclei were visualized by DAPI staining. Representative images of transfected cells were acquired using immunofluorescence microscope. (c) DU145 cells were cotransfected with plasmids as described in A for 72 h and then subjected to immunoprecipitation with anti-AR antibody, and followed by immunoblotting with anti-AR (IP) or anti-ubiquitin (IB) antibodies

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