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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Epithelial and interstitial Notch1 activity contributes to the myofibroblastic phenotype and fibrosis

Fig. 5

Notch1 signalling induces EMT and FMD by activating the TGF-β1/Smad2/3 pathway in vitro. a ELISA assay revealed that the levels of TGF-β1 in NRK-52E and NRK-49F cells were increased by Jag1-fc treatment. b Upregulated mRNA expression levels of TGF-β1 and TGF-β1R in Jag1-fc-treated NRK-52E and NRK-49F cells were strengthened by TGF-β1. c Increased expression and phosphorylation of Smad2 and Smad3 in Jag1-fc-treated NRK-52E and NRK-49F cells were enhanced by TGF-β1. d Upregulated Smad2/3 expression and nuclear localization in Jag1-fc-treated NRK-52E and NRK-49F cells were strengthened by TGF-β1. Bar = 20 μm. All data are presented as means ± SDs. Jag1-fc, 1 μg/ml; TGF-β1, 5 ng/ml. *P < 0.05, versus the control; #P < 0.05 versus the Jag1-fc-treated group

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Cell Communication and Signaling

ISSN: 1478-811X