Fig. 1
From: Targeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy
Validation of sgRNAs-mediated knockout targeting NR2F6 in primary lymphocytes. a Schematic of the sgRNA-targeting sites within the Nr2f6 genomic locus. The sgRNA-targeting sequence in red, the protospacer-adjacent motif (PAM) sequence in bold grey and flanking primer pairs (bold, underlined, italics). b Detection of sgRNA Cas9-mediated cleavage of Nr2f6 by PCR with the Takara kit on wildtype thymocytes for sgRNA.Nr2f6.03 and sgRNA.Nr2f6.04 including the kit control and untreated cells without cleavage. c T7 cleavage assay on genomic DNA isolated from CD3+ Cas9 transgenic T cells using sgRNA.Nr2f6.04 or combining sg. RNA.Nr2f6.04 and sg. RNA.Nr2f6.05 (sgRNA.Nr2f6.4.5) including a non targeting control (NTC) sg. RNA. Results shown are derived from at least two independent experiments. Untr., untreated, NTC, non-targeting control