Fig. 8From: The multi-site docking protein Grb2-associated binder 1 (Gab1) enhances interleukin-6-induced MAPK-pathway activation in an SHP2-, Grb2-, and time-dependent mannerGab1 signalling impacts IL-6-induced MAPK-dependent gene expression in a time-dependent manner. a HEK293, HEK293 Gab1-KO and HEK293 Gab1-rec cells were seeded and cultivated for 24 h. On the following day, cells were serum starved for 4 h and subsequently stimulated with Hy-IL-6 (50 ng/ml) for the times, indicated. Total mRNA was extracted and subjected to qRT-PCR analysis to monitor c-Fos mRNA expression. The expression of c-Fos mRNA was normalized to SDHA mRNA expression. Expression of mRNA is given in fold of mRNA expression in control (=untreated HEK293 cells, set as 1). Data are given as mean of three independent experiments ± SD. b Additionally, HEK293 cells were pre-treated with U0126 (10 μM) or its solvent control DMSO for 30 min prior to stimulation with Hy-IL-6 (50 ng/ml, 30 min). Total mRNA was extracted and subjected to qRT-PCR analysis to monitor c-Fos mRNA expression as described for (a). (c, d) SOCS3 mRNA expression was analysed as described for c-Fos mRNA in (a) and (b)Back to article page