Fig. 10

Gab1 signalling impacts IL-6-induced MAPK-dependent gene expression in normal human dermal fibroblasts in a time-in dependent manner. a Normal human dermal fibroblasts (NHDF) were seeded and cultivated for 24 h. On the following day, cells were serum starved for 24 h. After serum starvation, NHDF were pre-treated with Wortmannin (100 nM) or its solvent control DMSO for 30 min. Subsequently, cells were stimulated with Hy-IL-6 (50 ng/ml) for the times, indicated. Total mRNA was extracted and subjected to qRT-PCR analysis to monitor c-Fos mRNA expression. The expression of c-Fos mRNA was normalized to SDHA mRNA expression. Expression of mRNA is given in fold of mRNA expression in control (=untreated NHDF, set as 1). Data are given as mean of three independent experiments ± SD. b Additionally, NHDF were pre-treated with U0126 (10 μM) or its solvent control DMSO for 30 min prior to stimulation with Hy-IL-6 (50 ng/ml, 30 min). Total mRNA was extracted and subjected to qRT-PCR analysis to monitor c-Fos mRNA expression as described for (a). (c, d) SOCS3 mRNA expression was analysed as described for c-Fos mRNA in (a) and (b)