Fig. 5

BME treatment induces CHOP and ROS generation. a: Cell lysates from Cal27 and JHU022 with/without BME treatment were subjected to Western blot analysis for the CHOP using specific antibodies. The membrane was reprobed with antibody to actin as an internal control. b: Quantitative of Western blot band intensities using Image-J software. Small bar indicates standard error (*, p < 0.05; **, p < 0.01; *** p < 0.001). c: Cal27 and JHU022 with or without BME treatment were stained with mitoSox and flow cytometric analysis was performed to analyse mitochondrial ROS level at 510 nm. d: Schematic representation of lipogenesis pathway and probable mode of action of BME on regulation of phospholipids and thereby modulation ER stress and ROS associated cell death