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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Inhibition of the key metabolic pathways, glycolysis and lipogenesis, of oral cancer by bitter melon extract

Fig. 3

BME treatment inhibits expression of different enzymes of de novo lipogenesis. a: Relative mRNA expression of ACLY, ACC1 and FASN analysed by qRT-PCR in Cal27, and JHU022 cells with or without BME for 30 h. 18 s gene was used as internal control. b: Cell lysates from Cal27 and JHU022 with or without BME treatment for 30 h were subjected to Western blot analysis for ACLY, ACC1 and FASN using specific antibodies. The membrane was reprobed with antibody to actin as an internal control. c: Quantitative of Western blot band intensities using Image-J software. Small bar indicates standard error (*, p < 0.05; **, p < 0.01; *** p < 0.001). d: Schematic diagram showing different genes regulate lipogenesis and effect of BME on the genes

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