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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Inhibition of the key metabolic pathways, glycolysis and lipogenesis, of oral cancer by bitter melon extract

Fig. 1

BME treatment reduces expression of glycolytic genes. a: Relative mRNA expression of GLUT-1, PFKP, PKM, LDHA, and PDK3 was analysed by q-RT-PCR in Cal27 and JHU022 cells with/without BME. 18 s gene was used as internal control. b: Cell lysates from Cal27 and JHU022 with or without BME treatment for 30 h were subjected to Western blot analysis for GLUT-1, PFKP, LDHA, PKM and PDK3 using specific antibodies. The membrane was reprobed with antibody to actin as an internal control. c: Quantitative of Western blot band intensities using Image-J software. Small bar indicates standard error (*, p < 0.05; **, p < 0.01; *** p < 0.001). d: Schematic diagram showing different genes regulate glycolysis and effect of BME on the genes

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