Fig. 2From: Targeting PKCι-PAK1 signaling pathways in EGFR and KRAS mutant adenocarcinoma and lung squamous cell carcinomaCell viability by colony forming assay and western blotting by treatment using single reagent or combination of IPA-3 plus auranofin in HCC827 cell lines. IPA-3 plus auranofin potentiates colony formation inhibition and protein expression. a HCC827 cell colonies were grown under single or combination increasing dose of IPA-3 and/or auranofin. Fixed colonies were stained using crystal violet. The experiments were made at least three times and a representative image of colon formation assay is shown. b Concentration of crystal violet was represented as ratio to control (non-treatment colonies defined as 1). Crystal violet was absorbed using 2% sodium dodecyl sulfate and measured at 570 nm. c Combination index by each concentration of IPA-3 and auranofin. Combination index decreased in dose-dependent manner. Combination index was included in synergistic area (< 0.9). d HCC827 cell line was exposed to 50 μM IPA-3, 15 μM auranofin or 0.1 μM osimertinib and the combinations of IPA-3 plus auranofin or osimertinib for 6 h. Protein expression and activation were analyzed by western blotting. Actin was used as house-keeping protein. The experiments were made at least twice. Abbreviations: S, serine; T, threonine; Y, tyrosineBack to article page