Fig. 5

Cells expressing elevated levels of MYC are sensitive to LEF1 knockdown. a 5000 ARPE-19 cells with or without MYC overexpression were transfected with either control or LEF1 siRNA in 12-well plates for 7 days. Then the cells were stained with crystal violet, and the cell densities were quantified by using ImageJ. b ARPE-19 cells were extracted with NP40 lysing buffer, and the total cell lysates were subjected to Western blotting with the indicated antibodies. c-f 20,000 HCEC cells expressing control vector (c), truncated APC (d), KRas V12 (E), or P53 shRNA (F) were transfected with control, MYC, or LEF1 siRNA in 12-well plates for 7 days. Then the cells were stained with crystal violet, and the cell densities were quantified by using ImageJ. g 5000 DLD1 cells were transfected with control, MYC, or LEF1 siRNA in 12-well plates for 7 days, when cells were stained with crystal violet, and the cell densities were quantified by using ImageJ. h, i 5000 DLD1 (h) or RKO cells (i) were transfected with control or LEF1 siRNA in 12-well plates for 7 days. Then the cells were stained with crystal violet, and the cell densities were quantified by using ImageJ. j DLD1 cells were transfected with either control or LEF1 siRNAs for 3 days and then extracted with NP40 lysing buffer and subjected to Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001