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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation

Fig. 1

MYC regulates the expression of components of the WNT pathway. a Rat1 myc−/− fibroblasts expressing either vector or human MYC were collected and subjected for RNA-seq analysis. The heatmap was generated based on relative expression of each sample for the members of the WNT pathway regulated by MYC with cutoff of Log2FC = 0.585. Asterisks denote genes verified by using RT-qPCR or Western blotting. b RT-qPCR for the indicated genes in Rat1 fibroblasts myc−/− or Rat1 fibroblasts myc−/− reconstituted with human MYC. Expression levels of each gene was normalized to the levels of 18S, and the expression levels of each genes in myc−/− samples was set to 1. c-e Rat1 fibroblasts (c), NIH3T3 cells (d), and human colonic epithelial cells (HCEC, e) were extracted with NP40 lysing buffer, and the total cell lysates were subjected to Western blotting with the indicated antibodies. f Parental Rat1 fibroblasts with or without human MYC (hMYC) overexpression were transfected with control or MYC siRNAs for 3 days before protein extraction and Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001

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