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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: The PPAR-γ antagonist T007 inhibits RANKL-induced osteoclastogenesis and counteracts OVX-induced bone loss in mice

Fig. 7

Knock-down of PPARγ promotes osteoblastogenesis and the expression of osteoblast-specific gene expression in vitro. a-e Expression of the osteoblast-specific genes Runx2, OCN, OSX, ALP, COL1a, and OSX in osteoblasts following the transfection with two kinds of siRNA or the negative control. f ALP expression in osteoblasts cultured in the osteogenic medium and mineralized extracellular matrix was detected using Alizarin Red S staining treated after transfection with two kinds of siRNA or the negative control for 7 days and 21 days. g The number of ALP-positive cells and the OD values obtained for mineralized matrix solutions following transfected with siRNA or the negative control. h Osteoblasts were transfected with two kinds of siRNA or the negative control. Cell lysates were analyzed using western blotting. The expression of PPARγ, Runx2, and β-catenin was evaluated. All experiments were performed at least three times. *P < 0.05 and **P < 0.01 compared with the control group

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Cell Communication and Signaling

ISSN: 1478-811X