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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: CXCL1-LCN2 paracrine axis promotes progression of prostate cancer via the Src activation and epithelial-mesenchymal transition

Fig. 3

Prostate cancer cell-neutrophil interaction in transwell systems and the influence of LCN2 on tumor cells. a Representative cells migration images (magnification 40X) of PC-3 and DU145 co-cultured with neutrophil. b LCN2 secretion in neutrophil, tumor cell lines and co-cultured system were determined using ELISA. The LCN2 level of neutrophil control was normalized as 1. c MMP9/LCN2 secretion in tumor cell lines and respective co-cultured system with neutrophil were measured using ELISA. d Influence of LCN2 on prostate cancer cells proliferation was determined using SRB. DHT was used as control to treat androgen-sensitive cell line LNCaP and androgen-insensitive PC-3. e Representative cells migration images (magnification 40X) of PC-3 and DU145 treated with different doses of LCN2. f DU145 was treated with low concentration of CXCL1/LCN2 separately or in combination for 96 h. g Influence of LCN2 on benign prostate epithelial cell RWPE-1 proliferation was determined using SRB. h Cells migration of RWPE-1 treated with LCN2. Representative images (magnification 40X) and quantitative data were shown. Data are represented as mean ± SD of triplicates from a representative experiment of n = 3. Histograms shows quantitative results and p value was determined by ANOVA or t test, asterisks indicate *p < 0.05, **p < 0.01

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