Fig. 2

CXCL1 contributes to myofibroblast promoted migration in prostate cancer cells. a Representative images (magnification 40X) of cells migration in prostate cancer cells control and those co-cultured with WPMY-1 in transwell (TW) system. b CXCL1 secretion in PCa, naïve WPMY-1 and PCa CM treated WPMY-1 was determined using ELISA. This cytokine concentration was normalized with numbers of cells. c Influence of CXCL1 on prostate cancer cells proliferation was determined using SRB. DHT was used as control to treat androgen-sensitive cell line LNCaP and androgen-insensitive PC-3. d Representative cells migration images (magnification 40X) of PC-3 and DU145 treated with different concentrations of CXCL1. e Representative images (magnification 40X) of cells migration in prostate cancer cells control and those treated with CXCL1, with or without supplement of CXCR1/2 blockage using specific antibodies or inhibitor. f Influence of CXCL1 on benign prostate epithelial cell RWPE-1 proliferation was determined using SRB. g Representative cells migration images (magnification 40X) of RWPE-1 treated with CXCL1. Data are represented as mean ± SD from a representative triplicate experiment. Histograms shows quantitative results and p value was determined by ANOVA or t test, asterisks indicate *p < 0.05, **p < 0.01