Skip to main content

Advertisement

Table 3 The Effect of PTEN siRNA and CXCL12 on invasiveness of colon cancer cells

From: Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Cell line Relative number of invading cells (%)
PTEN siRNA Control siRNA LY294002 PTEN siRNA + CXCL12 (ng/ml)
1 10 100
HT-29 147.3 ± 16.7* 100 ± 11.3 70.6 ± 13.2* 144.7 ± 17.3 152.5 ± 17.3 186.0 ± 21.3*
WiDr 128.5 ± 9.63* 100 ± 12.0 68.1 ± 10.7* 127.2 ± 10.2 136.0 ± 16.3 176.3 ± 22.2*
CaCo-2 134.0 ± 18.0* 100 ± 16.4 61.3 ± 10.1* 136.2 ± 15.2 145.2 ± 12.8 153.7 ± 30.6*
Colo320 111.9 ± 9.14 100 ± 10.4 59.1 ± 10.1* 113.1 ± 20.3 122.6 ± 21.5 156.4 ± 15.0*
  1. NOTE: After transfection with PTEN siRNA or control siRNA, colon cancer cells were treated with different concentrations of CXCL12 and incubated for 24 h, followed by measurement of cell invasion by a Matrigel assay. Statistical significance was tested by one-way ANOVA followed by the Dunnett test. Statistical significance was inferred if p < 0.05. All data are expressed as mean ± s.d. *p < 0.01 when compared with the control siRNA group