Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Table 3 The Effect of PTEN siRNA and CXCL12 on invasiveness of colon cancer cells

Cell line	Relative number of invading cells (%)
	PTEN siRNA	Control siRNA	LY294002	PTEN siRNA + CXCL12 (ng/ml)
	PTEN siRNA	Control siRNA	LY294002	1	10	100
HT-29	147.3 ± 16.7*	100 ± 11.3	70.6 ± 13.2*	144.7 ± 17.3	152.5 ± 17.3	186.0 ± 21.3*
WiDr	128.5 ± 9.63*	100 ± 12.0	68.1 ± 10.7*	127.2 ± 10.2	136.0 ± 16.3	176.3 ± 22.2*
CaCo-2	134.0 ± 18.0*	100 ± 16.4	61.3 ± 10.1*	136.2 ± 15.2	145.2 ± 12.8	153.7 ± 30.6*
Colo320	111.9 ± 9.14	100 ± 10.4	59.1 ± 10.1*	113.1 ± 20.3	122.6 ± 21.5	156.4 ± 15.0*

NOTE: After transfection with PTEN siRNA or control siRNA, colon cancer cells were treated with different concentrations of CXCL12 and incubated for 24 h, followed by measurement of cell invasion by a Matrigel assay. Statistical significance was tested by one-way ANOVA followed by the Dunnett test. Statistical significance was inferred if p < 0.05. All data are expressed as mean ± s.d. *p < 0.01 when compared with the control siRNA group

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