Skip to main content

Advertisement

Table 1 Primer sequence and PCR condition

From: Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Gene name Primer sequences Tm(°C) Cycles Length(bp) Accession number
PTEN F: 5′-ACCAGGACCAGAGGAAACCT-3′
R: 5′-GCTAGCCTCTGGATTTGACG-3’
58 35 241 NM-000314
CXCL12 F: 5’-TTCCATTTGCAAGGGAAAAG-3′
R: 5′-ACACACAGCCAGTCAACGAG-3’
56 35 236 NM-000609
CXCR4 F: 5’-GAAGCTGTTGGCTGAAAAGG-3′
R: 5′-GAGTCGATGCTGATCCCAAT-3′
54 35 345 NM-003467