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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Fibroblast-derived CXCL12 regulates PTEN expression and is associated with the proliferation and invasion of colon cancer cells via PI3k/Akt signaling

Fig. 7

CXCL12-induced phosphorylation of Akt in human colon cancer cell lines. (a) The different concentration of CXCL12 activates phosphorylation of PI3K in HT-29, Colo32 0and CaC-2 cells. The cells were treated with different concentrations of CXCL12 (100 ng/ml) and cultured for for 0, 15, 30, or 60 min. The cells were gathered and lysed by lysis buffer. The 30 micrograms of lysed protein were used to do immunoblotting with a phospho-Akt antibody. Detection of total Akt levels served as a loading control. (b) Expression of phospho-Akt and total Akt in both PTEN siRNA treated and control siRNA treated HT-29 cells. (c) LY294002 inhibited Akt expression in untreated, PTEN siRNA transfected and control siRNA HT-29 cells

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